Effects of SPARC and Possible Receptors on Colon Cancer Cell Line

Objective: The aim of this study was to observe the apoptotic/cytotoxic effects exogenous SPARC on colon cancer cell line HT-29, then investigate function stabilin-1 and integrin αvβ3, which are possible receptors for in cells determine quantitation their receptor numbers.
 Methods: Appropriate doses it’s inhibitor, cilengitide added HT-29 were determined by xCELLigence Real-Time Cell Analysis system, SPARC-mediated caspase 3 expressions measured. Using RT-PCR gene expression levels SPARC, αvβ3 (silenced/nonsilenced with cilengitide) detected numbers per quantitated flow cytometry.
 Results: IC50 value as 4.57 μg/mL 50 nM. 5 caused increased apoptosis line. Significant increase observed group incubated contrarily, addition decreased expressions. numbers
 approximately 2-fold compared control. No significant changes stabilin-1.
 Conclusion: Exogenous shown reduce proliferation induce cells. Integrin is thought be mediating Quantification surface cell, we think have done first, can considered a marker follow-up anticancer treatments.